Comparative evaluation of microbial adhesion on porcelain subjected to two different surface treatments
Objective: To compare microbial adhesion on self glazed and overglazed surfaces of porcelain laminate veneer.
Methodology: Porcelain laminates made from IPS Empress were fabricated. These were subjected to two types of surface treatments, namely; overglaze and self glaze. A total of four laminate samples were fabricated viz. S1, S2, S3 and S4, of which S1 and S3 were overglazed and S2 and S4 were self glazed. Samples S1 and S2 were inoculated with streptococcus mutans for forty eight hours and S3 and S4 were inoculated with candida albicans for seventy two hours. 0.01 ml of microbial suspension was pipetted out from the specimens and incubated on specific growth agar media. This was left for 48 hours and then the colony forming units were counted. Scanning electron microscopic analysis was done to observe the micro-organisms on the laminate surface.
Results: The colony forming units on overglazed surfaces were less (S1 - 15x103 and S3 - 10x103) comparedto self glazed surfaces (S2 - 23x103 and S4 - 18x103).
Conclusion: It was concluded that the number of micro-organisms adhered to overglazed surfaces was less compared to self glazed surfaces.